Applications:Suitable for use in ELISA, Western and Dot Blot, Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:1000-1:5000Western Blot: 1:500-1:2500Immunohistochemistry: 1:200-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store at 4° C before opening.
DO NOT FREEZE.
This product is stable at 4° C as an undiluted liquid.
Dilute only prior to immediate use.
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
仕様
Size:500ug
Host:goat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, 50% glycerol, 10mg/ml BSA, and 0.01% sodium azide, pH 8.0. Labeled with alkaline phosphatase.
Specificity:RecognizesBiotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum and Biotinylated IgG.