Applications:Suitable for use in Immunomicroscopy, Flow Cytometry or FACS analysis.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 1:50 to 1:200 (~1ug will stain 1x10e6 cells)Optimal dilutions to be determined by the researcher.
Absorption Wavelength:490nm.
545nm, 565nm
Emission Wavelength:580nm
Label:R-Phycoerythrin (R-PE) MW: 240kD, from seaweed.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:500ug
Host:donkey
Source Antibody:sheep
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.02% sodium azide 40% glycerol. Labeled with PE.
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti phycoerythrin, anti donkey serum, sheep IgG and sheep serum. No reaction was observed against anti pepsin, anti donkey IgG Fc, or chicken, guinea pig, hamster, equine, human, mouse, rabbit or rat serum proteins.