Applications:Suitable for use in Flow Cytometry, Immunomicroscopy and FACS analysis.
Other applications have not been tested.
Recommended Dilution:Flow Cytometry: 1:100-1:250 (~1ug will stain 1x10e6 cells) Optimal dilutions to be determined by the researcher.
Label:R-Phycoerythrin (R-PE)Source: Seaweed
Molecular weight: 24kD.
Absorption Wavelength: 490nm, 545nm and 565nmEmission Wavelength: 580nm
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:500ug
Host:goat
Source Antibody:rat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied in as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.05% sodium azide. Labeled with R-Phycoerythrin (R-PE) MW 240kD. .Absorption Wavelength: 490nm, 545nm and 565nm. Emission Wavelength: 580nm. Reconstitute with 1ml sterile, ddH2O.
Specificity:Recognzies rat IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-phycoerythrin, anti-goat serum, rat IgG and rat serum. No crossreactivity was observed against anti-pepsin, anti-goat IgG F(c), or bovine, equine, human, mouse, rabbit and sheep serum proteins.