Applications:Suitable for FLISA, Western Blot, Immunomicroscopy and Flow Cytometry or FACS.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 1:100-1:250 (~1ug will stain 1x10e6 cells)Immunomicroscopy: 1:100-1:250Optimal dilutions to be determined by the researcher.
Excitation Wavelength: 488
Emission Wavelength: 575
Storage and Stability:May be stored at 4°C before opening.
DO NOT FREEZE! Stable at 4°C as an undiluted liquid.
Dilute only prior to immediate use.
Stable for 12 months at 4°C.
Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.
PE conjugates are sensitive to light.
仕様
Size：500ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with R-Phycoerythrin (PE) (MW 240kD).
Specificity：Recognizes mouse IgG F(c) fragment. Assay by IEP resulted in a single precipitin arc against anti-phycoerythrin, anti-goat serum, mouse IgG, mouse IgG F(c) and mouse serum. No reaction was observed against anti-pepsin, anti goat IgG F(c), mouse IgG F(ab’)2 or bovine, equine or human serum proteins.
