Applications:Suitable for use in FLISA and Immunofluorescence.
Other applications not tested
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Label: R-Phycoerythrin (R-PE) (Molecular Weight 240kD from seaweed)
Phycobiliprotein Concentration:0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound)
Absorption Wavelength: 490 nm, 545 nm and 565 nm
Emission Wavelength: 580 nm
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer
仕様
Size:500ug
Host:donkey
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc.
Form:Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitution: Reconstitute with 1ml distilled, deionized water.
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Mouse IgG and Mouse Serum. Minimum crossreactivity to Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Equine, Human, Rabbit, Rat and Sheep Serum Proteins