Applications:Suitable for use in Immunomicroscopy, Flow Cytometry, FACS, Immunofluorescence.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 1:50-1:200 (~1ug will stain 1x10e6 cells)Optimal dilutions to be determined by the researcher.
Label:R-Phycoerythin (R-PE) (MW 240kD) from seaweed.
Absorption:490, 545, 565nm
Emission:580nm
OD566nm/OD280nm:2.8
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:500ul
Host:donkey
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.6, 15mg/ml BSA (IgG and protease free), 0.05% sodium azide, 0.01% thimerosal, 40% glycerol.
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti phycoerythrin, anti donkey serum, human IgM, Human IgM Fc5u and human serum. No reaction against anti pepsin, anti donkey IgG F(c), bovine or equine serum proteins, or other human heavy or light chain proteins.