Applications:Suitable for use in ELISA, Western and Dot Blot, Immunohistochemistry, Immunomicroscopy.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：sheep
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.01% gentamicin sulfate, 40% glycerol. Labeled with horseradish peroxidase (HRP).
Specificity：Reactivity observed against Cy2, Cy3 and Cy5. Crossreactivity with other cyanine analog is unknown. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum, anti-peroxidase and cyanine coupled IgG.
Isotype：IgG
Calc Applications Abbrev：DB E IHC WB
Immunogen：A mixture of Cy2, Cy3 and Cy5 (KLH coupled)
