Applications:Suitable for use in ELISA, Immunoprecipitation, Western Blot, Immunohistochemistry, Immunodiffusion and Conjugation.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-1:50,000Western Blot: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:500ug
Host:sheep
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography
Form:Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide
Specificity:Recognizes Cyanine. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum and cyanine conjugated IgG. Reactivity was observed against Cy2, Cy3 and Cy5. Reactivity with other cyanine analogs is unknown.
Isotype:IgG
Calc Applications Abbrev:E IHC IP WB
Immunogen:A mixture of Cy3 and Cy5, conjugated to KLH