Applications:Suitable for use in FLISA and Immunofluorescence microscopy.
Other applications have not been tested.
Recommended Dilution:Immunofluorescence microscopy: 1:100-1:1000Optimal dilutions to be determined by the researcher.
Label:Cy5.29 (Cyanine 5.29-OSu) (MW: 975D)
Absorption Wavelength:650nm
Emission Wavelength:667nm
Fluorochrome/Protein Ratio:9.8 moles Cy5 per mole Donkey IgG
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:1mg
Host:donkey
Source Antibody:goat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc.
Form:Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease Free), 0.01% sodium azide. Reconstitute with 1ml sterile dH2O. Labeled with Cy5.
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-donkey serum, goat IgG and goat serum. No reaction was observed against chicken, guinea pig, hamster, equine, mouse, rabbit & rat serum proteins.