Application(s): suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays.
Recommended Dilution:ELISA: 1:10,000 to 1:100,000Optimal dilutions to be determined by the researcher.
Storage and Stability: May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:rabbit
Grade:Highly Purified
Purity:Purified from monospecific antiserum by delipidation, fractionation and ion exchange chromatography.
Form:Supplied as a lyophilized powder in PBS, pH 7.2, BSA. gentamicin sulfate.
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Lima Bean].