Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains.
Trypsin is inhibited by organophosphorous compounds such as diisopropylfluorophosphate and porcine-derived inhibitors.
Used in protein sequencing and tissue disassociation.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 1:5000-1:25,000Western Blot: 1:500-1:3000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：bovine
Grade：Highly Purified
Purity：Purified by delipidation, fractionation and ion exchange chromatography.
Form：Supplied as a liqud in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free). 0.01% sodium azide. Labeled with Biotin.
Specificity：Recognizes bovine Trypsin. Assay by IEP resulted in a single precipitin arc against anti-biotin, anti-rabbit serum as well as purified and partially purified Trypsin (Bovine pancreas).
