84-1025-32　［取扱停止］Ribonuclease A (RNase A, Pancreatic Ribonuclease, Rib 1, Ribonuclease A Family 1, Ribonuclease Pancreatic, Ribonuclease RNase A Family 1, Ribonuclease RNase A Family 1 Pancreatic, RNAseA, RNase 1, RNASE1, RNS1) (Biotin) 100ug　R2011-20

特徴

• Ribonuclease A is used to isolate DNA for RNA-free DNA.
• Ribonuclease A hydrolyzes RNA at C and U residues, cleaving between the 3’-Phosphate group of a pyrimidine nucleotide and the 5’-hydroxyl group of the adjacent nucleotide, i.e., those cleaving cyclic 2',3'-pyrimidine nucleotide residues either singly or at the termination of purine nucleotide chains (Volkin and Cohn 1953).
• The composition and structure of ribonuclease A has been extensively investigated.
• The tertiary structure was reported by Kartha, et al.
• (1967) and Lin, (1970).
• Wang, et al.
• (1975) indicate that flexibility in a three-dimensional structure is essential for optimal catalytic activity.
• Ribonuclease is inhibited by heavy metal ions and is competitively inhibited by DNA.
• The effect of denatured DNA is much greater than that of the native nucleic acid (Sekine et al.
• 1969).
• Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:15000-1:20,000Western Blot: 1:500-1:5000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
• Label:Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
• Biotin/Protein Ratio:10-20 BAC molecules/bovine pancreas IgG molecule
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.