84-1025-31　［取扱停止］Ribonuclease A (RNase A, Pancreatic Ribonuclease, Rib 1, Ribonuclease A Family 1, Ribonuclease Pancreatic, Ribonuclease RNase A Family 1, Ribonuclease RNase A Family 1 Pancreatic, RNAseA, RNase 1, RNASE1, RNS1) 10mg　R2011-13

特徴

• Ribonuclease A is used to isolate DNA for RNA-free DNA.
• Ribonuclease A hydrolyzes RNA at C and U residues, cleaving between the 3’-Phosphate group of a pyrimidine nucleotide and the 5’-hydroxyl group of the adjacent nucleotide, i.e., those cleaving cyclic 2',3'-pyrimidine nucleotide residues either singly or at the termination of purine nucleotide chains (Volkin and Cohn 1953).
• The composition and structure of ribonuclease A has been extensively investigated.
• The tertiary structure was reported by Kartha, et al.
• (1967) and Lin, (1970).
• Wang, et al.
• (1975) indicate that flexibility in a three-dimensional structure is essential for optimal catalytic activity.
• Ribonuclease is inhibited by heavy metal ions and is competitively inhibited by DNA.
• The effect of denatured DNA is much greater than that of the native nucleic acid (Sekine et al.
• 1969).
• Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Conjugation.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:40,000-1:80,000 use 1ug of R2011-13 in a standard sandwich ELISA using I1904-46Q and ABTS as a substrate for 30 min at RT
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.