Horseradish Peroxidase (HRP) is a hemoprotein catalyzing the oxidation by hydrogen peroxide of a number of substrates such as ascorbate, ferrocyanide, cytochrome C and the leuko form of many dyes.
Horseradish Peroxidase has been found well suited for the preparation of enzyme conjugated antibodies, due in part to its ability to yield chromogenic products and to its relatively good stability characteristics.
Applications:Suitable for use in ELISA, Dot Blot, Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:10,000-1:40,000Western Blot: 1:1000-1:4000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Host：sheep
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added.
Specificity：Recognizes horseradish peroxidase. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Peroxidase (Horseradish).
