HRP is widely used as an enzymatic label in immunoassays.
Usually, the enzyme is coupled to antibodies, lectins or haptens.
Coupling to antibodies etc.
may be performed through the carbohydrate side chains of the HRP.
HRP consists of the basic isoenzyme having a molecular weight of 44kD.
Applications:Suitable for use in ELISA, Western Blot, Dot Blot, and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000-1:20,000Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Host：mouse
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes horseradish peroxidase. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified horseradish peroxidase (HRP).
