Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1;2000-1:10,000Western Blot: 1:500-1:2500Immunohistochemistry: 1:250-1:1250Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:goat
Source Antibody:porcine
Grade:Highly Purified
Purity:Purified by delipidation, salt fractionation and ion exchange chromatography.
Form:Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Reconstitute with 100ul of sterile dH2O. Labeled with Horseradish peroxidase (HRP).
Specificity:Recognizes porcine pepsin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxide, anti-goat serum and purified and partially purified pepsin (Porcine Stomach)