Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression.
Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP.
Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly.
This light can be detected by a luminometer.
The total light emission is proportional to the luciferase activity of the sample.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:50,000-1:200,000.
Assayed against 1ug of Luciferase [Photinus pyralis (Firefly)] in a standard capture ELISA using ABTS (A0030) as a substrate for 30 minutes at RT
Western Blot: 1:5000-1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Labeled with horseradish peroxidase (HRP).
Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
HRP conjugates are sensitive to light.
仕様
Size：1mg
Host：goat
Grade：Highly Purified
Purity：Purified from monospecific antiserum by delipidation, fractionation and ion exchange chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% gentamicin sulfate. Labeled with HRP.
Specificity：Recognizes Luciferase. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum and purified and partially purified Luciferase [Photinus pyralis (Firefly)]. No reactivity is observed against Sea pansy (Renilla reniformis) luciferase.
