The 26kD GST (glutathione S-Transferase) domain, encoded by the parasite Schistosoma japonicum, is easily purified by affinity chromatography on immobilized glutathione.
For this reason, numerous prokaryotic expression vectors have been developed allowing research to fuse recombinant protein purification.
GST is utilized as a fusion protein in protein expression from a range of prokaryotic expression vectors, including the pGEX family of vectors.
Antibodies against GST will detect the expressed GST protein or a GST-fusion protein, and can be utilized for immuno-purification of GST-fusion proteins.
Application(s): suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.
Storage and Stability: May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：2ml
Host：goat
Grade：Serum
Purity：Purified by delipidation and defibrination.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].
