Since its discovery as an antibiotic (shown subsequently to be due to peroxide formation) there has been an interest in glucose oxidase, chiefly because of its utility in glucose estimation.
For most clinical work the crude form of the A.
niger enzyme has been satisfactory.
However, it contains trace amount of polysaccharidases such as amylase, maltase and sucrase which can contribute to falsely high glucose levels.
The purified enzyme is free of these traces and is recommended for analytical use in the presence of di-or polysaccharides.
Glucose analytical systems employ the enzyme utilizing H2O2 production reacting with Fe(CN)-in the presence of luminol to produce luminescence proportional to the initial glucose concentration.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and conjugation.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000 to 1:10,000, assayed against 1ug of Glucose Oxidase using HRP conjugated rabbit anti sheep IgG and ABTS as a substrate for 30 min at RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：10mg
Host：sheep
Grade：Highly Purified
Purity：Purified by delipidation, fractionation and ion exchange chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes Glucose Oxidase. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum as well as purified and partially purified Glucose Oxidase (Aspergillus niger).
