Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000-1:25,000.
Assayed against 1ug of Glucose Dehydrogenase in a standard ELISA using I1904-46Q, IgG, H&L (HRP) and ABTS (A0030) as a substrate for 30 minutes at RT.
Western Blot: 1:500-1:3000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:10mg
Host:goat
Grade:Highly Purified
Purity:Purified from monospecific antiserum by delipidation, fractionation and ion exchange chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity:Recognizes Glucose Dehydrogenase. Crossreacts with Bacillus. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glucose Dehydrogenase (Bacillus species).