Applications:Suitable for use in Western Blot, ELISA, and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:1000-1:4000Western Blot: 1:1,000 - 1:5,000Immunohistochemistry: 1:500 - 1:2,500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:rabbit
Source Antibody:bovine
Grade:Highly Purified
Purity:Purified by delipidation, fractionation and ion exchange chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 0.01% gentamicin sulfate, 10mg/ml BSA (IgG and Protease free), 40% glycerol. Labeled with Horseradish Peroxidase (HRP).
Specificity:Recognizes Carbonic Anhydrase II. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Carbonic Anhydrase II (Bovine Erythrocytes).
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Bo
Immunogen:Carbonic Anhydrase II (Bovine Erythrocytes).