Bilirubin Oxidase antibody is an enzyme that increases the oxidation rate of bilirubin to biliverdin and other substrates.
It is ideal for investigators interested in Metabolism or Signal Transduction research.
Applications:Suitable for Western Blot, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000-1:5000Western Blot: 1:500-1:2500Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:goat
Grade:Highly Purified
Purity:Purified from monospecific antiserum by delipidation, fractionation and ion exchange chromatography.
Form:Supplied as a lyophilized powder in PBS, pH 7.2, 0.01% sodium azide. Reconstitute with 100ul sterile dH2O.
Specificity:Recognizes Myrothecium verrucaria Bilirubin Oxidase. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Bilirubin Oxidase [Myrothecium verrucaria].