Beta-2-microglobulin (B2M) is found in the serum of normal individuals and in the urine in elevated amounts in patients with Wilson disease, cadmium poisoning, and other conditions leading to renal tubular dysfunction.
Like immunoglobulins, prealbumin, and the beta protein found in the amyloid of Alzheimer disease, B2M has a predominantly beta-pleated sheet structure that may adopt the fibrillar configuration of amyloid in certain pathologic states.
The protein is a single polypeptide chain of molecular weight 11,600 (human 119 aa, chromosome 15).
B2M associates with heavy chain of class I MHC antigens on the cell surface.
A transient complex of MHC-heavy chain and B2M is is known to be assembled into the TAP molecule involving interaction with a number of chaperones.
Binding of the processed peptide releases the class I-B2M complex to the cell surface.
Absence of binding leads to degradation in the proteasome.
Progressive hepatic iron overload, indistinguishable from that observed in HFE, was found only in mice homozygous for the mutated B2M gene.
Applications:Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000-1:5000ELISA: 1:2000-1:10,000Immunohistochemistry: 1:500-1:3000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by delipidation, salt fractionation and ion exchange chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Reconstitute with 100ul sterile ddH2O. Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule. Labeled with Biotin.
Specificity：Recognizes human beta-2-Microglobulin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, as well as purified and partially purified beta-2-Microglobulin (human urine).
