Applications:Suitable for use in ELISA, Western Blot..
Other applications not tested.
Recommended Dilution:ELISA: 1:7,000 to 1:26,000.
Against 1ug of Aspartate Transaminase (AST) [Pig Heart] in a standard sandwich ELISA using I1904-46Q IgG, H&L (HRP) Pab Gt x Rb and A0030 ABTS Stable Liquid Substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:10mg
Host:sheep
Source Antibody:porcine
Grade:Highly Purified
Purity:Purified from monospecific antiserum by delipidation, fractionation and ion exchange chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Aspartate Transaminase (AST) [Porcine Heart].