Applications:Suitable for Immunoblotting (western or dot blot), ELISA, Immunoperoxidase electron microscop and Immunohistochemistry, as well as other peroxidase-antibody based enzymatic assays.
Other applications not tested.
Recommended Dilution:ELISA: 1:200-1:1200.
Assayed against 1ug of Asparaginase (E.
coli) in a standard capture ELISA using ABTS as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer
仕様
Size：100ug
Host：rabbit
Grade：Highly Purified
Purity：Purified from monospecific antiserum by delipidation, fractionation and ion exchange chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, containing 10mg/ml BSA and 0.01% gentamicin sulfate, before the addition of glycerol to 40%. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes Asparaginase. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Asparaginase [E. coli].
