D-Amino Acid Oxidase is a flavoprotein oxidase, flavin being the only prosthetic group.
The enzyme from porcine kidney has been extensively studied.
It is isolated as a stable crystalline complex with benzoate, from which the holo and apoenzyme may be prepared.
The benzoate is readily exchanged for a substrate.
Application:Suitable for use in Western Blot, ELISA and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000-1:20,000Western Blot: 1:500-1:5000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：sheep
Source Antibody：porcine
Grade：Highly Purified
Purity：Purified from monospecific antiserum by delipidation, fractionation and ion exchange chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes porcine D-Amino Acid Oxidase. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-sheep serum as well as purified and partially purified D-Amino Acid Oxidase (porcine kidney).
