Applications: Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000 to 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:sheep
Source Antibody:bovine
Grade:Highly Purified
Purity:Purified from monospecific antiserum by delipidation, fractionation and ion exchange chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added.
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified alkaline phosphatase [calf intestine] and anti-sheep serum. Crossreactivity against alkaline phosphatase from other tissues and species may occur but have not been specifically determined.
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Bo
Immunogen:Alkaline Phosphatase from calf intestine