Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:3000 to 1:15,000 (Coat with 1ug of alkaline phosphatase in a standard sandwich ELISA)
Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:rabbit
Source Antibody:bovine
Grade:Highly Purified
Purity:Purified by delipidation, fractionation and ion exchange chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide, 40% glycerol. No stabilizing protein added.
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti rabbit serum as well as purified and partially purified alkaline phosphatase (calf intestine). Crossreactivity against alkaline phosphatase from ohter tissues and species may occur, but have not been determined.
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Bo
Immunogen:Alkaline Phosphatase from calf intestine