Applications:Suitable for Immunoblot (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunoblot: 1:1000-1:5000.
Assayed using 1ug Alkaline Phosphatase and T6000: TMB Stable Liquid Substrate 1.13mM/Liter for HRPO (3,3',5,5'-Tetramethylbenzidine)
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by delipidation, salt fractionation and ion exchange chromatography.
Form：Supplied in as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate, 40% glycerol. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human alkaline phosphotase. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as wel as purified and partially purified alkaline phosphatase (human intestine). Crossreactivity against alkaline phosphatase from other tissues and species may occur but have not been specifically determined.
Isotype：IgG
Calc Applications Abbrev：DB E IHC WB
Calc Crossreactivity：Hu
Immunogen：Alkaline Phosphatase from human intestine
