Alkaline phosphatase (AP) is a ubiquitously expressed enzyme which removes phosphate groups from target molecules, including DNA, RNA and alkaloids, under alkaline conditions, and is present at higher concentrations in the placenta (placental AP), intestines (intestinal AP) and liver/bone/kidney (tissue non-specific AP).
Although the exact biochemical function of BAP is uncertain, measurement of the serum levels of BAP can be used as a biochemical indicator of bone turnover.
Conditions which present with a decrease in the level of BAP (hypophosphatasemia) include the inherited bone-deforming disorder hypophosphatasia and osteoporosis, whilst an increase in BAP (hyperphosphatasemia) is associated with Paget’s disease, bone fractures and osteosarcomas.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 1:4000- 1:125,000Western Blot: 1:500- 1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10mg
Host：rabbit
Grade：Highly Purified
Purity：Purified from monospecific antiserum by delipidation, fractionation and ion exchange chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes Phosphatase, Alkaline. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum as well as purified and partially purified Alkaline Phosphatase [E. coli].
