Applications:Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:4000 to 1:16,000 using 1.0 ug of Aldolase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio:10–20 BAC molecules per Rabbit Muscle IgG molecule
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：rabbit
Grade：Highly Purified
Purity：Purified by delipidation, fractionation and ion exchange chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, (IgG and Protease free) 0.01% sodium azide, 40% glycerol. Conjugated with Biotin.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified rabbit muscle aldolase, anti-biotin and anti-goat serum.
