Applications:Suitable for use in Western Blot and ELISA.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:500-1:5000.
ELISA: 1:5000-1:20,000.
Assayed against 1ug of rabbit muscle Aldolase in a standard ELISA using a Rabbit xGt IgG (H&L) (HRP) and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) (A0030) as a substrate for 30 minutes at RT
Immunoprecipitation: 1:100.
~5ul immunopreciptiates 50ul of protein lysate
Optimal working dilutions must be determined by the end user.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：rabbit
Grade：Highly Purified
Purity：Purified by delipidation, salt fractionation and Ion Exchange chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes rabbit Aldolase. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum and purified and partially purified rabbut muscle Aldolase. Species Crossreactivity: Human
