ALT requires pyridoxal-5-phosphate (PLP) as prosthetic group.
ALT was previously known as serum glutamic pyruvic transaminase (SGPT).
ALT is a cytoplasmic enzyme that catalyzes the transamination of alpha-ketoglutarate and L-alanine, forming glutamate and pyruvate.
ALT is found in liver, kidney, brain and heart, as two isoforms, cytosolic ALT and mitochondrial ALT.
These enzymes function in cellular nitrogen metabolism and also liver gluconeogenesis.
The highest activities of ALT are found in hepatocytes and striated (skeletal and cardiac) muscle cells.
Therefore, increased serum ALT activity can accompany hepatocellular injury or necrosis of striated muscle.
With cell injury or death, ALT (a 'leakage' enzyme) escapes from the cytosol.
Determination of ALT activity is a relatively sensitive indicator of hepatic damage in certain animal species and can help determine whether further diagnostic tests.
Applications:Suitable for use in Western Blot, Dot Blot, ELISA, Immunohistochemistry and Conjugation.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000-1:3000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Also Available:A1210-05: Alanine Transaminase (ALT) Pab Sh xPoA1210-06: Alanine Transaminase (ALT) Pab Sh xPoA1210-15: Alanine Transaminase (ALT) (HRP) Pab Sh xPoA1210-20: A1210-15: Alanine Transaminase (ALT) (Biotin) Pab Sh xPo
仕様
Size：100ug
Host：sheep
Source Antibody：porcine
Grade：Highly Purified
Purity：Purified by delipidation, fractionation and ion exchange chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.01% gentamicin sulfate, before the addition of 40% glycerol. Label with Horseradish Peroxidase (HRP).
Specificity：Recognizes Alanine Transaminase. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-sheep serum as well as purified and partially purified Alanine transaminase (ALT) (Porcine Heart). Species Crossreactivity: Reported to crossreact with bovine ALT in Western Blot.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Bo Po
Immunogen：Alanine Transaminase (ALT) from Porcine Heart.
