Applications:Suitable for ELISA, Western Blot and Immunohistochemistry and Conjugation.
Other applications not tested.
Recommended Dilution:ELISA: 1:20,000-1:80,000.
Assayed against 1ug Acid Phosphatase in standard sandwich ELISA using IgG, H&L (HRP) Pab Gt xRb (Catalog No: I1904-46Q) and ABTS Stable Liquid Substrate 1.46mM/Liter for HRPO (Catalog No: A0030)
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Also available:A0560: Acid Phosphatase (Potato) Pab Rb x (antiserum)A0560-05: Acid Phosphatase (Potato) Pab Rb x (Highly purified)A0560-06: Acid Phosphatase (Potato) (Biotin) Pab Rb xA0560-07: Acid Phosphatase (Potato) (HRP) Pab Rb x
仕様
Size:100ug
Host:rabbit
Grade:Highly Purified
Purity:Purified by delipidation, fractionation and ion exchange chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide, 40% glcyerol. No stabilizing proteins added.
Specificity:Recognizes Acid Phosphatase (Potato). Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum as well as purified and partially purified Acid Phosphatase (Potato).