Applications:Suitable for use in Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy.
Other applications not tested.
Recommended Dilution:ELISA (capture): 1:4000 to 1:20,000 using peroxidase conjugated streptavidin and ABTS as a substrate for 30 min at RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:1mg
Host:rabbit
Source Antibody:human
Grade:Highly Purified
Purity:Purified by delipidation, fractionation and ion exchange chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free) 0.01% sodium azide, 40% glycerol.Labeled with Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10–20 BAC molecules per Rabbit IgG molecule.
Specificity:Recognizes human a-1-Acid Glycoprotein. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum as well as purified and partially purified a-1-Acid Glycoprotein [Human Plasma].
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Hu
Immunogen:a-1-Acid Glycoprotein from human plasma.