Applications:Suitable for use in Immunofluorescence Microscopy, Fluorescent Western Blot and FLISA.
Other applications not tested.
Recommended Dilutions:Immunofluorescence Microscopy: 1:1000-1:5000FLISA: 1:10,000-1:50,000Optimal dilutions to be determined by the researcher.
Absorption Wavelength:550nm
Emission Wavelength:570nm
Fluorochrome/Protein Ratio:2.5 moles TRITC per mole of Rabbit IgG
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Tetramethylrhodamine isothiocyanante (TRITC) (MW 444D).
Specificity：Recognizes mouse IgA. Assay by IEP resulted in a single precipitin arc against anti-rabbit serum, mouse IgA and mouse serum. Specificity was confirmed by ELISA at <1% crossreactivity against other mouse heavy or light chain isotypes..
