Applications:Suitable for use in Immunofluorescence Microscopy, FLISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Immunofluorescence Microscopy: 1:1000-1:5000FLISA: 1:10,000-1:50,000Optimal dilutions to be determined by the researcher.
Fluorochrome/Protein Ratio:2.0 moles per mole of Rabbit IgG
Absorption Wavelength:550nm
Emission Wavelength:570nm
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Rhodamine labeled products are light sensitive.
仕様
Size：500ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with TRITC.
Specificity：Recognizes human IgM (Fc5mu) fragment. Assay by IEP resulted in a single precipitin arc against anti-rabbit serum, human IgM and human serum. Specificity was confirmed by ELISA minimal crossreactivity against other human heavy or light chain isotypes.
