Applications:Suitable for use in ELISA, Western Blot, Dot Blot, Immunoperoxidase Electron Microscopy and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000 to 1:12,000.
Assayed against 1ug of feline IgG in a standard capture ELISA using ABTS (A0030) as a substrate for 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:2mg
Host:goat
Source Antibody:feline
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate, 40% glycerol. Labeled with horseradish peroxidase (HRP).
Specificity:Assay by IEP resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Feline IgG, Feline IgG F(c) and Feline Serum. No reaction was observed against Feline IgG F(ab’)2.