Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:ELISA: 1:2000-1:50,000Western Blot: 1:1000-1:10,000Immunohistochemistry: 1:500-1:2500Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months at -20°C after receipt.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1.5mg
Host：rabbit
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml polyethlene glycol (PEG-8000), 0.01% gentamicin sulfate. Labeled with Horseradish peroxidase (HRP). Reconstitute with 1mll sterile ddH2O.
Specificity：Recognizes IgG, FC. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, bovine IgG, bovine IgG F(c) and bovine serum. No reaction was observed against bovine IgG F(ab’)2.
