Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-1:200,000Western Blot: 1:5000-1:100,000Immunohistochemistry: 1:500-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months at -20°C after receipt.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：2ml
Host：goat
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a powder lyophilized from a solution containing PBS, pH 7.2, 15mg/ml BSA. Reconstitute with 2ml sterile ddH2O. Labeled with Horseradish peroxidase (HRP).
Specificity：Recognizes bovine IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum and bovine serum.
