Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration.
Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI).
Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle.
When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I.
The letter “I” is given due to its inhibitory character.
The tissue specific subtypes are:Slow-twitch skeletal muscle isoform troponin I, TNNI1 (1q31.3, 191042)Fast-twitch skeletal muscle isoform troponin I, TNNI2 (11p15.5, 191043)Cardiac troponin I, TNNI3 (19q13.4, 191044)
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Specificity：Recognizes human cardiac Troponin I. Recognizes epitopes located in the 27-39 region of Troponin I. Crossreactivity with human skeletal Troponin I is 0.02% by ELISA.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu
Immunogen：Highly purified human cardiac Troponin I
