Human brain natriuretic peptide (BNP) is a secreted protein which is a member of the natriuretic peptide family.
BNP is a cardiac hormone, which is synthesised as a pro-hormone (proBNP), and is proteolytically cleaved to release a biologically active fragment (BNP), and an inactive fragment (NT-proBNP) into the circulation.
BNP is predominantly secreted from the cardiac ventricles in response to volume and pressure overload, and results in a number of biological activities including natriuresis, diuresis, vasorelaxation, and inhibition of the sympathetic nervous system.
A high concentration of BNP in the bloodstream is indicative of heart failure.
Applications:Suitable for use in RIA, ELISA, Immunohistochemistry, Immunocytochemistry and Indirect Immunofluorescence.
Stains rat brain sections using 3% paraformaldehyde and indirect immunofluorescent staining.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with 100ul sterile PBS, 10mg/ml BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Neat serum
Form：Supplied as a lyophilized powder. No preservatives added. Reconstitute with 100ul sterile PBS, 10mg/ml BSA.
Specificity：Recognizes human BNP (100%). Does not crossreact with human Atrial Natriuretic Peptide, ACTH (human, 1-39), human Calcitonin, or Vasoactive Intestinal Peptide. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：E IC IF IHC RIA
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to human Brain Natriuretic Polypeptide covalently attached to bovine thyroglobulin.
