Applications:Suitable for use in Immunofluorescence, ELISA and Immunohistochemistry.
Also suitable for immunostaining of extra- or intracellular components in light microscopy.
Other applications not tested.
Recommended Dilution:Indirect Immunofluorescence: 1:40 for frozen bovine liver and skin tissue with fluorescein anti-rabbit IgG conjugate
ELISA: 1:100Immunohistochemistry: 1:250 on fixed bovine paraffin sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ul
Host：rabbit
Source Antibody：bovine
Grade：Highly Purified
Purity：Purified by ion exchange chromatography.
Form：Supplied as a lyophilized powder. No preservative added. Reconstitute in 500ul sterile distilled water.
Specificity：Recognizes bovine Collagen Type IV. Does not crossreact with bovine Collagen Types I, II, III, IX, XI; bovine Fibronectin or other species type IV Collagens.
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Bo
Immunogen：Collagen Type IV extracted and purified from bovine lens capsule
