Alzheimer’s Disease (AD) is a neurodegenerative disorder characterized by progressive loss of memory and cognition in the elderly.
One of the most important and initial step involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43 aa peptides (beta amyloid1–40, 1–42, and 1–43).
Polymerization of b-amyloid (Ab) and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition.
Ab deposits have also been found to contain 2 additional proteins termed a-synuclein and b-synuclein.
The 140 aa a-synucleins is identical with non-Ab component (NACP) of AD.
The 134 aa b-synuclein is homologous to 14kD bovine phosphoneuroprotein 14.
Mutations in a-synuclein gene causing a replacement of alanine with a threonine has the potential to cause the protein to misfold.
Synucleins are primarily expressed in the brain.
At least 3 forms, two large (140 aa SYN-1 & 149 aa SYN-2) and a small form (SYN-3, 42 aa) are produced by alternative splicing.
Recently a new isoform, termed g-synuclein (SNCG) or breast cancer gene 1 (BCG1) has been cloned (human 127 aa, rat/mouse 123 aa).
All three synucleins shown ~40% identity.
g-synuclein is primarily expressed in brain (substantia nigra), and over expressed in advanced infiltrating carcinoma of breast, and in ovarian cancer.
Applications: Suitable for use in ELISA, Western Blotting, Immunoprecipitation.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:100,000 using 50–100ng of control peptide/wellWestern Blotting: 1:1000-1:5000Immunoprecipitation: 5–10ul neat serum or 1–10ug affinity pure antibody per 100ug of tissue
Optimal dilutions to be determined by the researcher.
Control peptides are available to confirm specificity of antibodies.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Neat Serum
Form：Undiluted, neat serum, preservative added
Specificity：The rat peptide sequence is 100% conserved in mouse g-synuclein. No significant sequence homology is seen with either human g-synuclein or synucleins-alpha or beta) or other proteins. We recommend the use of another antibody for human g-synuclein that is made using the human sequence.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Rt
Immunogen：18-aa peptide near the C-terminus of rat g-synuclein coupled with KLH
