Alzheimer’s Disease (AD) is a neurodegenerative disorder characterized by progressive loss of memory and cognition in the elderly.
One of the most important and initial step involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43aa peptides (beta amyloid1–40, 1–42, and 1–43).
Polymerization of b-amyloid (Ab) and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition.
Ab deposits have also been found to contain 2 additional proteins termed a-synuclein and b-synuclein.
The 140 aa a-synucleins is identical with non-Ab component (NACP) of AD.
The 134aa b-synuclein is homologous to 14kD bovine phosphoneuroprotein 14.
Mutations in a-synuclein gene causing a replacement of alanine with a threonine has the potential to cause the protein to misfold.
Synucleins are primarily expressed in the brain.
At least 3 forms, two large (140aa SYN-1 & 149 aa SYN-2) and a small form (SYN-3, 42 aa) are produced by alternative splicing.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/wellWestern Blot: 1-10 ug/ml using Chemiluminescence techniqueOptimal dilutions to be determined by the researcher.
Control Peptide:S9501-05P Synuclein, beta Human CT Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by antigen affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
Specificity：Recognizes human Synuclein. The peptide sequence corresponding to human beta-synuclein (134 aa). Species sequence homology: rat beta-synuclein (137 aa) and bovine PNP14 (134 aa). It has no appreciable homology with synuclein-alpha or other proteins.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Sunthetic 15 aa peptide located near the C-terminus of human synuclein-beta (KLH).
