Alzheimer’s Disease (AD) is a neurodegenerative disorder characterized by progressive loss of memory and cognition in the elderly.
An early step involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43aa peptides (beta amyloid1-40, 1-42, and 1-43).
Polymerization of b-amyloid (Ab) and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition.
Ab deposits have also been found to contain 2 additional proteins termed a-synuclein and b-synuclein.
The 140aa a-synuclein is identical with non-Ab component (NACP) of AD.
The 134aa b-synuclein is homologous to 14kD bovine phosphoneuroprotein 14.
Mutations in a-synuclein gene causing a replacement of alanine with a threonine has the potential to cause the protein to misfold.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/wellWestern Blot: 1-10ug/ml
Optimal dilutions to be determined by the researcher.
Control Peptide: S9500-21P
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.
Specificity：Recognizes human a-Synuclein CT. No significant homology is seen with beta-synuclein or with other known proteins. Species sequence homology: Rat, mouse and chicken: 86%
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 15aa located near the C-terminus of human synuclein-alpha.
