84-1022-25　Interleukin 22R, NT (IL-22R NT, CRF2–9, IL-TIF R1, IL-10 Related T Cell-derived Inducible Factor) 100ug　I8443-12

特徴

• A novel cytokine, designated IL-TIF for IL-10 related T cell-derived inducible factor and IL-22, was recently identified (1–3).
• The receptor for IL-22 (IL-22R, also termed CRF2–9 and IL-TIF-R1 chain) is a new member of the class II cytokine receptor family (3,4).
• IL-22R forms a complex with IL-10 receptor b chain and mediates IL-22 signaling.
• IL-22 and its receptor activate JAK-STAT signaling pathway.
• IL22R is expressed in normal liver and kidney and their cell lines HepG2 and TK-10.
• A soluble form of IL-22 receptor, also termed IL-22 binding protein (IL-22BP) and IL-22 receptor-a 2 (IL-22RA2), was identified very recently (5–7).
• IL-22BP prevents binding of IL-22 to the functional cell surface IL-22R complex and neutralizes IL-22 activity.
• LPS induces IL-22 expression, which indicates the role of IL-22 in inflammatory response.
• Applications: Suitable for use in Western Blotting.
• Other applications not tested.
• Recommended Dilution: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
• Human HepG2 cell lysate can be used as positive control.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.