A novel cytokine related to IL-2 and IL-15 was recently identified and designated IL-21 (1).
The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family (1,2).
IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling (3,4).
Both IL-21R and the gc are necessary for the IL-21 function.
IL-21 and its receptor activate JAK-STAT signaling pathway.
IL-21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines.
IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
Applications:Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlImmunohistochemistry (paraffin): 10ug/mlImmunofluorescence: 20ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Raji Cell Lysate and Rat Lung Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes human Interleukin-21R. An approximately 60kD band can be detected. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide (NITDQSGNYSQECGS) corresponding to amino acids 97 to 111 of human IL-21R precursor.
