Dendritic cells (DCs) that control immune responses were recently found to capture and transport HIV from the mucosal area to remote lymph nodes, where DCs hand over HIV to CD4+ T lymphocytes.
DCs also amplify the amount of virus and extend the duration of viral infectivity.
Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC-SIGN.
ICAM-3 is the natural ligand for DC-SIGN.
A DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC-SIGN2) was identified recently.
DC-SIGN forms a novel gene family with DC-SIGNR and many alternatively spliced isoforms of DC-SIGN and DC-SIGNR.
The expression of DC-SIGN was found in mucosal tissues including placenta, small intestine, and rectum.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/ml.
A band at ~44kD can be detected
Optimal dilutions to be determined by the researcher.
Positive Control:Human placenta lysate.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human DC-SIGN.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa277-293 (CYFMSNSQRNWHDSITA) of human DC-SIGN.
