The 26kD GST (glutathione S-Transferase) domain, encoded by the parasite Schistosoma japonicum, is easily purified by affinity chromatography on immobilized glutathione.
For this reason, numerous prokaryotic expression vectors have been developed allowing research to fuse recombinant protein purification.
GST is utilized as a fusion protein in protein expression from a range of prokaryotic expression vectors, including the pGEX family of vectors.
Antibodies against GST will detect the expressed GST protein or a GST-fusion protein, and can be utilized for immuno-purification of GST-fusion proteins.
Applications:Suitable for use in FLISA, Immunofluorescence, and Western Blot.
Other applications not tested.
Recommended Dilutions:FLISA: >1:20,000Western Blot: >1:10,000Immunofluorescence: 1:500-1:2500Optimal dilutions to be determined by the researcher.
Absorption Wavelength: 495nm
Emission Wavelength: 528nm
Fluorochrome/Protein Ratio: 3.6moles FITC per mole of goat IgG
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
FITC conjugates are sensitive to light.
仕様
Size：500ug
Host：goat
Grade：Highly Purified
Purity：Purified by Ion exchange chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with FITC.
Specificity：Recognizes Glutathione S Transferase, Schistosoma japonicum. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].
